How to collect larvae of Amblycheila cylindriformis

Amblycheila cylindriformis larval burrow | Major Co., Oklahoma

Amblycheila cylindriformis larval burrow | Major Co., Oklahoma

Step 1. Go to your favorite grassland habitat in the western half of the Great Plains anywhere from Texas north to South Dakota and look for barren soil amongst the vegetation. Clay banks near streams or in ravines and even vertical clay bluff faces are also good (although I have not myself observed the latter). “My” spot is in the Glass Mountains of northwestern Oklahoma, where talus slopes in mixed-grass prairie beneath flat-topped mesas and the ravines that cut through them provide just enough slope for this species’ liking.

Burrow diameter of ~8mm identifies this as a 3rd instar larva.

Burrow diameter of ~8mm identifies this as a 3rd instar larva.

Step 2. Look for large, almost perfectly round burrow entrances that go straight down from the surface. By large, I mean approximately 6–8 mm in diameter—as large a burrow as any tiger beetle in North America will make. Many other insects create burrows, but tiger beetle burrows are generally recognizable by their almost perfectly circular shape and clean, beveled edge. Look closely, and the burrow will be seen to actually be slightly D-shaped to match the shape of the tiger beetle larva’s head—the large, sickle-shaped, upward-facing jaws resting against the flat part of the D. In the case of this species, they tend to be found in clusters of several burrows in close proximity to each other. The burrow in these photos was found at the upper edge of a drainage ravine on the upper part of the talus slopes (see diagram in this post).

Dig around the burrow, carefully excavating along the grass stem, until the larva is reached.

Dig around the burrow, carefully excavating along the grass stem, until the larva is reached.

Step 3. Try this first—chew the end of a long, narrow grass stem (frayed and sticky will be easier for the larva to grab hold of) and stick it down the burrow until it hits bottom, tap lightly a few times to entice a bite, then yank (and I mean yank!) the stem out. With luck, the larva will come flying out of the burrow and land somewhere on the ground in front of you. (By the way, if you have never done this, you are missing one of the greatest treats that insect collecting has to offer. If you have done it, you owe it to yourself to show this to somebody else who has not ever seen it—their shocked reaction at the sight of the flying larva is beyond priceless!) Larvae are not always in the mood to bite, however, so if the so-called “fishing” technique does not work then you will have to dig. Stick the grass stem back down the burrow and begin excavating around the burrow, carefully prying away the soil adjacent to the burrow to prevent it from falling into and obscuring the burrow. Keep excavating as you follow the grass stem down until, at least, you reach the larva. In the photo above you can see in the lower right-center area the burrow with the grass stem protruding from it and the larva placed on a clump of soil in front of the shovel (for sense of scale). It seems I had an easy time of it with this larva, as literature sources report larval burrows extending down to depths of a meter or more.

Amblycheila cylindriformis 3rd instar larva.

Amblycheila cylindriformis 3rd instar larva.

Step 4. Behold the beast! There is nothing more that can be said—these larvae are ginormous! This particular larva measured a full 62 mm from the tips of its mandibles to the tip of its abdomen—that’s 2½ inches! No other tiger beetle larva in North America reaches this size, except perhaps the related A. hoversoni (South Texas Giant Tiger Beetle).

The distinctly smaller 2nd pair of eyes confirm this is not Tetracha or Cicindela (sensu lato)...

The distinctly smaller 2nd pair of eyes confirm this is not Tetracha or Cicindela (sensu lato)…

Step 5. If size alone isn’t enough, you can confirm that the larvae does indeed belong to the genus Amblycheila by looking at its eyes—their are two pairs, and the 1st pair (closest to the mandibles) are distinctly larger than the 2nd pair. This isn’t clearly visible in the photo above because I doused the larva with water to remove the mud and dirt that encrusted it upon removal from its burrow.

...and the well-separated hooks on the 5th abdominal segment confirm it is Amblycheila.

…and the distinctly separated hooks on the 5th abdominal segment confirm it is Amblycheila.

Step 6. Another way to distinguish larvae of the genus Amblycheila is by looking at the hooks on the hump of the 5th abdominal segment, best done with a hand lens (or, even better, with an MP-E65 lens!). All tiger beetle larvae have several pairs of large hooks that the larva uses to brace itself against the wall of its burrow when capturing prey to prevent the struggling prey from pulling the tiger beetle larva out of its burrow. Larvae in the genus Omus, restricted to the Pacific region of North America, have three pairs of hooks (referred to as the outer, middle, and inner hooks), while all other North American tiger beetle genera have two (having lost the outer pair). In Amblycheila and Tetracha the hooks are simple and thornlike, while larvae of all other North American genera have much longer middle hooks that are curved and sickle-shaped (e.g., Cylindera celeripes in this post). Amblycheila larvae can be distinguished from Tetracha larvae by the middle and inner hooks on each side being distinctly separated rather than touching at the base (e.g., Tetracha floridana in this post). There is also a cluster of short, stout hairs around the base of each hook in Amblycheila that is missing in Tetracha (e.g., Tetracha virginica in this post).

The numerous stout setae are also characteristic of the genus.

The numerous stout setae are also characteristic of the genus.

Step 7. Lastly, don’t forget to look at the hump in lateral profile—it is as alien a structure as any in the insect world. In the case of Amblycheila larvae, the bed of hairs posterior to the hooks is comprised of much shorter, stouter, and more densely placed hairs than larvae of Tetracha.

Copyright © Ted C. MacRae 2013

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About Ted C. MacRae

Ted C. MacRae is a research entomologist by vocation and beetle taxonomist by avocation. Areas of expertise in the latter include worldwide jewel beetles (Buprestidae) and North American longhorned beetles (Cerambycidae). More recent work has focused on North American tiger beetles (Cicindelidae) and their distribution, ecology, and conservation.
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18 Responses to How to collect larvae of Amblycheila cylindriformis

  1. Ted, this was a great post. I’m a Tiger Beetle nut too and even reared a few when i was younger. But I’d love to see them snagged out of the burrow, as you described. That sounds too fun and hopefully, sometime I’ll get to a habitat where this species occurs and have the opp to try and fish for them… Thanks for sharing.

  2. Mike Powell says:

    Fishing for larvae? It sounds like the pilot episode for a new television series.

  3. Fishing for tiger beetle larvae sounds like way too much fun! Great blog, as usual…

    EC

  4. forkinriver says:

    Great post. I enjoyed your level of detail and accompanying photos. I feel compelled to go scout some Omus and do some grass fishing.

  5. Ben Coulter says:

    Is that hemolymph by the edge of the pronotum, or did it vomit?

    • Yes, hemolymph. I knicked it while digging – wasn’t sure if I’d find another one so took the photos before pickling it. Fortunately I was able to dig up another one w/o injury and am trying to rear it to adulthood.

      • Ben Coulter says:

        That sounds cool. Do you have any pics of the enclosure? How deep does the substrate need to be?

        • Just a large “Critter Carrier” filled with native soil to a depth of ~8 inches. I don’t know if it will work or not – I’ve already got another larva collected as a 2nd instar in 2009 that has made it to 3rd instar but spends months at a time with the burrow plugged. When it does open the burrow it is ravenous!

          • Ben Coulter says:

            They sure look like they would be! I wonder how long the species spends as a larva? At that size, I would guess at least 4 years, maybe more. It would be cool if the 2009 one would emerge as an adult one of these days.

            • Amblycheila have been reared in the lab (A. cylindriformis and A. hoversoni, for example); however, I can’t seem to find any published information with details of the technique. I’m just going by what little information I’ve been able to glean from these sources. Contrary to what they have said, A. cylindriformis adults definitely do make burrows and spend much of their time in the burrows.

              There will definitely be photos if either the 2009 or 2012 larvae emerge as an adult!

  6. Jon Quist says:

    What a great idea for a post! I’d love to do one in any way related to A. schwarzi once I stumble upon the first habitat. At the Bean Museum in Utah, I recall seeing many specimens… I’m regreting not writing down their localites as it is one of the more uncommon species.

  7. What an interesting blog! Tiger Beetle larvae are truly bizarre. I saw them in Costa Rica (Monteverde Reserve) in an almost vertical sandy slope along a dirt road. Their heads were in the holes, which lacked the beveled edges of this one. The heads were flat against the surface and almost invisible with the flatness of the heads and the dirt on them. It was night. We dug one out to see the whole larva and the hump and hooks. They are so vicious. I wonder what they can catch to eat on a vertical area. Did your larvae ever pupate? What does it look like? Did the adults emerge and how long did it take? I was interested in the comments above that a Tiger Beetle larva might take 2 or more years to mature and that they plug their holes for long periods, even months. I was also interested in what you said about Amblychelia cylindriformis adults making burrows. I had never heard before that adult Tiger Beetles make burrows. What do adult burrows look like? I wish we had tried the bite-grab-yank technique on the ones in Costa Rica!

    • Hi Lauren. I ended up pickling the 2012 larvae, but the 2009 larva ended up pupating last year—unbeknownst to me, as the burrow stayed plugged all summer long. I figured it had died and set the container aside, but this spring a beautiful, fully-formed adult emerged—nearly four years after I collected it as a 2nd-instar larva!

      Lots of adult tiger beetles make burrows, especially those that live in sandy habitats (see my post on Cicindela arenicola for an example). This is the first “clay” species that I’ve seen doing it, and because of their large size the burrows are also quite large, with ragged openings that look nothing like the perfectly-shaped larval burrows.

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